Main Organiser

Julius Centre University of Malaya

Co-organiser

Department of Social and Preventive Medicine, Faculty of Medicine, University of Malay

Supported by

University of Malaya

IFN GAMMA AND IL-10 PLASMA AS AN INFLAMMATION MAKER ON CHILD LIVING WITH ACTIVE ADULT TUBERCULOSIS.

Author

Pudji Lestari 1 , Sahiratmadja E 2 ; Endaryanto A 3

Institution

1. Public Health Department, Faculty of Medicine, Airlangga University Surabaya, Indonesia
2. Health Research Unit, Faculty of Medicine Padjadjaran University Bandung
Indonesia 3. Pediatric Department, Faculty of Medicine Airlangga University Surabaya, Indonesia

Abstract

Objectives: This research aimed to explore immune response on children living with active adult TB case in associations with infected and disease development.

Methods: In a cross-sectional study design, children living with active TB adult for at least 8 weeks were recruited. Clinical examination, TST, and chest X ray were performed on these children. They classified as TB class 1 (TST negative), and TB class 2 (TST positive >10 cm), and TB class 3 if child had clinical sign. Since, the PBMC’s 16SrRNA PCR all positive, in 43 subsamples, they also classified as ‘sick’ or not ‘sick’ according to clinical sign only, regardless the TST result. Plasma was separated from the blood sample, IFN gamma and IL-10 was measured with ELISA in a sub sample children.

Results: Of 56 index cases with active TB adult, 73 contact TB children were recruited, 41 and 37 could be cytokines measured for IFN gamma and IL10 respectively. IFN gamma tends to be higher on TB class 3, as IL-10 variation between classes was little. The ratio of IFN gamma /IL10 was significantly different between classes TB. The ratio also differs significantly between ‘sick’ and ‘not sick’ group.

Conclusion: In children living with active TB patient, IFN gamma and IL-10 plasma could reflect disease development. This finding could be considered as an alternative on diagnosis pediatric tuberculosis.