Main Organiser

Julius Centre University of Malaya

Co-organiser

Department of Social and Preventive Medicine, Faculty of Medicine, University of Malay

Supported by

University of Malaya

ANTIPROLIFERATIVE ACTIVITY OF BRUCEA JAVANICA AND APOPTOSIS DEATH IN HUMAN BREAST CARCINOMA, MCF-7.

Author

Abdullah NR, Nordin FJ, Afzan A, Sidek HM, Omar O, Ibrahim N

Institution

National University of Malaysia

Abstract

Objectives: To determine the antiproliferative activity of B.javanica extract and evaluate the apoptosis effect on human breast carcinoma.

Methods: SRB assay was used to determine the antiproliferative activities against a panel of human breast carcinoma cell lines, MCF-7, MDA MB 231, BT-549, SKBR3 and T47D. Hoechst 33258 nuclear staining was done to study the apoptosis morphology.

Results: The extract showed more potential to inhibit MCF-7 proliferation with IC50, 0.381 ± 0.123 µg/ml when compared with other cell lines. Therefore, MCF-7 was selected for further study on its mechanism of action. This extract exhibit less cytotoxicity effect toward normal cell line, MDBK with IC50 >2 µg/ml. MCF-7 cells treated with B.javanica demonstrated DNA fragmentation, nuclear condensation and cell shrinkage. The apoptotic index was 0.570 ± 0.667%, 59.024 ± 5.421%, 66.710 ± 5.626%, 89.954 ± 3.527%, 91.786 ± 5.937% and 99.045 ± 1.537% after exposure to B.javanica at 0, 0.25, 0.50, 1.00, 2.00, and 4.00 µg/ml, respectively. The increasing percentage of cells undergone apoptosis cell death showed that this extract induced apoptosis to MCF-7 cell line in dose-dependent manner.

Conclusion: B.javanica exhibit antiproliferative activity against human breast carcinoma, MCF-7 by inducing apoptotic cell death. Further study is demanded to elucidate its detail mechanism of action.