Main Organiser

Julius Centre University of Malaya

Co-organiser

Department of Social and Preventive Medicine, Faculty of Medicine, University of Malay

Supported by

University of Malaya

ANALYSIS OF GENE EXPRESSION IN THE OFFSPRING FROM ONE TYPE2 DIABETIC PARENT WITH IMPAIRED GLUCOSE TOLEREANCE

Author

Qvist R, Chinna K , Bee J

Institution

Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Julius Centre University Malaya, Kuala Lumpur

Abstract

Objectives: Type 2 diabetes is known to result from the interaction of genetic and environmental factors, and the predisposition to develop this disease is strongly determined by genetic factors. Offspring of type 2 diabetic patients are at a great risk of developing the disease. Peripheral blood cells play an important role in diabetes, yet very little is known about the molecular mechanisms of PBC’S regulation in insulin homeostasis

Methods: In this study we explored the profiles of 89 genes using the human diabetic PCR array.

Results: Gene expression profiling of mRNA from PBCs, from 3 first degree relatives with IGT, 3 first degree relatives with NGT, 3 freshly diagnosed type 2 diabetics and one normal control using a PCR array with 89 diabetic genes revealed differential expression of 42 genes with fold change of 2 and above either in the positive or negative directions. These genes included genes in the insulin signaling pathway, insulin secretion, enzymes in the carbohydrate metabolism, inflammation and innate immunity.

Conclusion: Gene expression changes in PBCs could distinguish the progression from NGT to type 2 diabetes. Identification of candidate genes in peripheral blood could provide easily accessible biomarkers to monitor the progression to type 2 diabetes in first degree relatives of type 2 diabetic parents