Main Organiser

Julius Centre University of Malaya

Co-organiser

Department of Social and Preventive Medicine, Faculty of Medicine, University of Malay

Supported by

University of Malaya

PREVALENCE OF HEPATITIS C VIRUS GENOTYPES IN THE NORTHERN OF IRAN (MAZANDARAN) FROM 2009 TO 2011

Author

Mohammadreza Haghshenas

Institution

Molecular Cell-Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Abstract

Objectives: HCV has been classified into six major genotypes and multiple subtypes. Prevalence of HCV genotypes are most distributed among of areas in the worldwide. In this study the genotype of HCV were identified in HCV positive patients by Real Time Polymerase Chain Reaction (RT-PCR).

Methods: HCV- positive serum samples of 86 patients with chronic hepatitis from 2009 to 2011 were studied. HCV-RNA was extracted from plasma samples using Qiagen RNeasy Mini Kit. Plasma samples from HCV positive patients were confirmed the presence of HCV nucleic acid and determined the genotypes of HCV genome by RT- PCR using the PureLink Viral RNA/DNA Kits (Invitrogen) and Amp-license Russia genotype kit with type of specific primers and probs. All samples were examined in the virology laboratory of Sari Medical School.

Results: The mean age of patients were 39+/- 14.3 (range, 18 to 72) year that 74 (86%) patients were male and 12 (14%) were female. The mean average serum level of AST and ALT was 89.5 and 103.7 IU/lit. In this study, 58.2% had genotype 3a and 58.2% had genotype 1a/b.

Conclusion: This study indicates that the genotype 3a is the most frequent followed by the genotype 1a/b in our region. Prevalence and incidence of HCV genotypes are with distributed among of areas and different genotypes show different responses with alpha interferon therapy. So, it is very important to determine the predominant subtypes of HCV.