Main Organiser

Julius Centre University of Malaya

Co-organiser

Department of Social and Preventive Medicine, Faculty of Medicine, University of Malay

Supported by

University of Malaya

OXIDATIVE DNA DAMAGE, TOTAL ANTIOXIDANT CAPACITY AND ADIPOSITY IN PREGNANCY: A LONGITUDINAL STUDY

Author

Loy See Ling 1 , Sirajudeen KNS 2 , Hamid Jan Jan Mohamed 1

Institution

1 Nutrition Programme, School of Health Sciences, Universiti Sains Malaysia, Malaysia
2 Department of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia, Malaysia

Abstract

Objectives: The metabolic changes and exaggeration of oxidative stress during pregnancy may contribute to the development of maternal obesity. Hence, we aimed to evaluate changes in the levels of oxidative DNA damage, total antioxidant capacity and lipid profile during pregnancy and to correlate the associations of these parameters with maternal total body fat mass.

Methods: Fasting blood samples of 159 women with uncomplicated pregnancies were collected at 15-22 weeks and 32-39 weeks of gestation to carry out the biochemical parameters in this longitudinal study. Maternal total body fat mass was assessed using bio-impedance analysis.

Results: Pregnant women at 32-39 weeks of gestation showed a significantly higher levels of cholesterol (P<0.001), triglyceride (P<0.001), DNA damage (P<0.001) and total antioxidant capacity (P<0.001) compared to 15-22 weeks of gestation. Pearson correlation indicated a positive association of maternal total body fat mass with triglyceride (P=0.010), DNA damage (P<0.001) and total antioxidant capacity (P<0.001) at 15-22 weeks of gestation. However, only DNA damage remained positively associated with maternal total body fat mass at 32-39 weeks of gestation. A significant association was noted between DNA damage and total antioxidant capacity in both periods.

Conclusion: Maternal adiposity is associated with elevated oxidative DNA damage which induces a compensatory increase of the total antioxidant capacity during pregnancy.