Main Organiser

Julius Centre University of Malaya

Co-organiser

Department of Social and Preventive Medicine, Faculty of Medicine, University of Malay

Supported by

University of Malaya

HISTOLOGICAL OBSERVATION OF THE HIPPOCAMPUS AND FRONTAL CORTEX OF EXPERIMENTAL SPRAGUE-DAWLEY RATS FED WITH LEAD IN A DOSE RELATED MANNER

Author

N.A.W. Abd. Aziz, M.F. Yahaya, S.L Teoh, A.A. Latiff, S. Das, T.A. Kamarudin

Institution

Universiti Kebangsaan Malaysia (UKM)

Abstract

Objectives: During recent times, lead poisoning cases have been on the rise. The present study aimed to look into the histological changes occurring in the brain following lead ingestion in a dose-related manner.

Methods: Eighteen male Sprague-Dawley rats weighing 150-200g were randomly divided into 3 groups. A control (CTRL) group was fed with distilled water. The PB2 and PB4 groups were fed with 0.2% and 0.4% lead acetate in distilled water respectively, for 30 days. The hippocampus and frontal cortex were subjected to light microscopic examination.

Results: It was observed that there was apoptosis on neurons of the hippocampus and frontal cortex occurred in a dose-related manner after 30 days of lead ingestion. Compared to CTRL, PB2 showed the presence of apoptotic neurons accompanied with disorganized neuronal cells. PB4 showed a significant increase of apoptotic cells in both hippocampus and frontal cortex. However, significant histological changes between the 2 experimental groups were not observed.

Conclusion: Lead ingestion in a dose-related manner is capable of causing histopathological changes in the hippocampus and frontal cortex in rats. The cause of lead toxicity is to be borne in mind in day-to date life where one runs the risk of getting exposed to lead.