Main Organiser

Julius Centre University of Malaya

Co-organiser

Department of Social and Preventive Medicine, Faculty of Medicine, University of Malay

Supported by

University of Malaya

CARDIOPROTECTIVE EFFECT OF LAGENARIA SICERARIA (MOLINA) STANDL. ON MYOCARDIAL APOPTOSIS IN EXPERIMENTALLY INDUCED MYOCARDIAL INFARCTION IN WINSTAR ALBINO RATS

Author

Vijayakumar M 1 , Selvi V 2 , Krishnakumari S 2 , Noorlidah A 1 , Priya K 3 , Ahmed ABA 1

Institution

1 Institute of Biological Science, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
2 Department of Biochemistry, Kongunadu Arts and Science, Coimbatore, Tamilnadu, India
3 Department of Biochemistry, PSG College of Arts and Science, Coimbatore, Tamilnadu, India

Abstract

Objectives: The present study was designed to scientifically evaluate the cardioprotective potential (in vivo and in vitro) of Lagenaria siceraria (Molina) Standl. fruits on isoproterenol-induced myocardial infarction in wistar albino rats.

Methods: Lagenaria siceraria (Molina) Standl. fruit extract (LSFE) was administered orally to wistar albino rats in three different doses (100, 250 and 500 mg kg-1 LSFE), 6 days a week for 5 weeks. At the end of this period, all the rats, except the normal untreated rats that served as the control group, were administered Isoproterenol, 85 mg kg-1 subcutaneously, for 2 consecutive days to induce myocardial injury. After 48 h, rats were anaesthetized with anesthetic ether, sacrificed and the levels of marker enzymes, anti-apoptotic effect and histological observations of the heart tissues were performed.

Results: Our results suggest that of LSFE maintained the entire baseline groups showed no significant alterations in serum marker enzyme levels in comparison to that of control group, which in the absence of any cellular injury (as evidenced by histological studies), is considered to be non-lethal and the LSFE significantly increases the number of viable cells, prevents DNA fragmentation and decreases the number of apoptotic cells. From this study shows that 500 mg kg-1 of LSFE protected against ISO-induced myocardial injury.

Conclusion: This study demonstrates that LSFE has a significant effect in the protection of heart against myocardial infarction induced by isoproterenol. We believe that LSFE may contribute to developing novel strategies in the prevention and treatment of cardiotoxic effects of elevated levels of catecholamine.